ABSTRACT
By consulting literatures of traditional Chinese medicine(TCM), comparing data about the morphological description of several species from genus of Salvia, and combining the biological studies in resources,this paper made a textual research for the origin,name,medicinal history,functional dominance and folk application Salviae Yunnanensis Radix. This study aimed to provide a reference for further studies and utilization of Salviae Yunnanensis Radix. The findings revealed that the origin of Zidanshen recorded in ancient Chinese herbal literatures was S. yunnanensis,which were widely used for amenorrhea,dysmenorrhea,irregular menstruation,tumescent pain,limb numbness and many kinds of blood stasis. Relevant clinical application and modern pharmacological evidences of Salviae Yunnanensis Radix are mutually supportive,which indicates that TCM based on clinical experience has a high development value. The herbal literatures revealed that Salviae Yunnanensia Radix has a high research and development value in gynecology,blood stasis and other fields. We shall fully excavate folk experience in clinical application of Salviae Yunnanensis Radix. On this basis, we shall further strengthen relevant researches on material basis,pharmacodynamics and mechanism,in order to promote the utilization of resources and product development of Salviae Yunnanensis Radix.
ABSTRACT
Objective To explore the application of DNA barcode technology in identification of ethnic medicine Salvia yunnanensis and its related species, so as to provide reference for identification of S. yunnanensis. Methods A DNA barcode database of S. yunnanensis and its adulterants was successfully constructed. Data were analyzed by aligning sequences, analyzing variation sites, calculating genetic distances, and establishing Neighbor-Joining trees. The database was used to identify 45 S. yunnanensis decoction piece samples purchased from drug stores. Results The results showed that the ITS2 barcodes can be used to identify S. yunnanensis and its adulterants. Conclusion The use of DNA barcoding for market supervision can broaden the application of this technology and provide an excellent inspection method for the supervisory institutions. This study substantially contributes to the protection of public health worldwide.
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Objective: To study the protective effects and the mechanism of Salvia yunnanensis extract on hypoxia/reoxygenation injury in cultured rat H9c2 cardiomyocytes.Methods: The hypoxia/reoxygen (H/R) injury model was established in H9c2 cell strain with or without the extract of Salvia yunnanensis.The cultured H9c2 cardiomyocytes were randomly divided into 6 groups: the normal control (C) group, H/R group, H/R+verapamil (H/R+V) group, H/R+Salvia yunnanensis extract at low dose (H/R+L, 0.01 mg·L-1) group, medium dose (H/R+M, 0.1 mg·L-1) group and high dose (H/R+H, 1.0 mg·L-1) group.The cell viability was measured by MTT assay and the activity of malondialdehyde (MDA) and lactate dehydrogenase (LDH) was measured by a detection kit.Fluorescence absorbance (A) value was measured by a fluoroscopy to show the intracellular reactive oxygen species (ROS) levels.Results: Compared with that in the model group, the survival rate of myocardial cells was significantly higher in Salvia yunnanensis extract at low, medium and high dose groups (P＜0.05 or P＜0.01), and the intracellular LDH leakage (P＜0.05 or P＜0.01), the content of MDA in cytoplasm (P＜0.01) and the intracellular ROS levels significantly decreased in Salvia yunnanensis extract at high dose group (P＜0.05).Conclusion: The extract of Salvia yunnanensis has protective effect on hypoxia/reoxygenation injury in cultured rat H9c2 cardiomyocytes, and the mechanism may be related to the reduction of lipid peroxides and removal of cell oxygen free radicals.
ABSTRACT
To study the fingerprints of liposoluble components in Salvia yunnanensis roots collected from different habi-tats, and compare with the fingerprints of Salvia miltiorrhiza. Methods: The fingerprints were determined by HPLC and the results were analyzed by a similarity evaluation system for chromatographic fingerprints of TCM ( Version 2004 A) . Results:The fingerprints of different samples of Salvia yunnanensis roots showed high similarity. And the characteristic peaks in the fingerprints of the liposoluble components were basically the same as those of Salvia miltiorrhiza, while the contents of several main effective components were lower than those in Salvia miltiorrhiza. Conclusion: The liposoluble components in Salvia yunnanensis roots show relatively high similarity with those in Salvia miltiorrhiza, however, there are obvious differences in the contents of the main constituents.